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誘電泳動による微粒子操作を応用した迅速な免疫測定
https://doi.org/10.15099/00006555
https://doi.org/10.15099/00006555735a13a1-eb98-4831-b42f-f2b1b02344c6
名前 / ファイル | ライセンス | アクション |
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01-01_annual_report33_page001to007.pdf (469.3 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2014-07-11 | |||||
タイトル | ||||||
タイトル | 誘電泳動による微粒子操作を応用した迅速な免疫測定 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 誘電泳動 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | dielectrophoresis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DEP | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 微粒子操作 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 免疫測定 | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.15099/00006555 | |||||
ID登録タイプ | JaLC | |||||
著者 |
安川, 智之
× 安川, 智之 |
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著者別名 | ||||||
姓名 | Yasukawa, Tomoyuki | |||||
その他(別言語等)のタイトル | ||||||
その他のタイトル | Rapid immunosensing based on dielectrophoretic manipulation of particles | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A rapid immunosensing method for measuring two tumor markers, alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA), based on particle manipulation by negative dielectrophoresis (n-DEP) was developed. When microparticles modified with different antibodies were subjected to n-DEP manipulation by the application of an AC voltage, they accumulated in particular positions in poly(dimethylsiloxane) (PDMS) fluidic channels modified with corresponding antibodies within 1 min. The presence of a specific antigen, AFP or CEA, allowed the irreversible capture of microparticles via the formation of immuno-complexes. Uncaptured microparticles redispersed after the AC voltage was switched off. The fluorescent intensity from the irreversibly captured microparticles allowed us to determine the concentration of AFP and CEA in the sample. The range for both AFP and CEA assays was 0.1-100 ng/mL, which was sufficient to cover the concentration required for medical diagnoses. Our system measured the concentrations of AFP and CEA simultaneously in a single device with two channels modified for different antibodies. Since n-DEP was used for the rapid manipulation of the microparticles toward the PDMS surface, the time required for the assay was substantially short: 1 min for forcing and 5 min for redispersion of microparticles and sensing. | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 富山大学水素同位体科学研究センター研究報告, 33巻, 2014.06.30, pp.1-7 | |||||
書誌情報 |
富山大学水素同位体科学研究センター研究報告 巻 33, p. 1-7, 発行日 2014-06-30 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13463675 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11841872 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
国立国会図書館分類 | ||||||
主題Scheme | NDLC | |||||
主題 | ZP8 | |||||
出版者 | ||||||
出版者 | 富山大学水素同位体科学研究センター | |||||
資源タイプ(DSpace) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article |