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The system has two distinct experimental chambers: a tritium circulation system and an exhaust-gas detritiation device. The former consists of tritium storage, purification and gas chromatographic hydrogen isotope separation units. The performance of the isotope separation unit was examined for 20 Pa･ｌ, a sample gas mixture containing protium, deuterium and tritium under the conditions of operation temperature at 77 K and carrier-gas (Ne) flow of 50 cc/min. Six isotope compounds of hydrogen were reproducibly separated within an adequate period of time (within 5000 seconds). 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