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Expression, purification, and crystallization of a plant polyketide cyclase from Cannabis sativa
http://hdl.handle.net/10110/00018117
http://hdl.handle.net/10110/0001811734b295d4-1eb8-4ed6-a9e1-448518b602aa
名前 / ファイル | ライセンス | アクション |
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ACFSBC_71(12)_1470to1474_Yang (170.3 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-02-07 | |||||
タイトル | ||||||
タイトル | Expression, purification, and crystallization of a plant polyketide cyclase from Cannabis sativa | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cannabinoids | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cannabis sativa | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cyclase\Olivetolic acid | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Yang, Xinmei
× Yang, Xinmei× Matsui, Takashi× Mori, Takahiro× Taura, Futoshi× Noguchi, Hiroshi× Abe, Ikuro× Morita, Hiroyuki |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Plant polyketides are a structurally diverse family of natural products. In the biosynthesis of plant polyketides, the construction of the carbocyclic scaffolds is a key step for diversifying the polyketide structure. Olivetolic acid cyclase (OAC) from Cannabis sativa L. is the only known plant polyketide cyclase that catalyzes the C2/C7 intramolecular aldol cyclization of linear pentyl tetra-β-ketide CoA to generate olivetolic acid in the biosynthesis of cannabinoid. The enzyme is also thought to belong to the dimeric α+β barrel (DABB) protein family. However, because of lack of the functional analysis of the other plant DABB proteins and low sequence identity with the functionally and structually characterized bacterial DABB proteins, the catalytic mechanism of OAC has remained unclear. To clarify the intimate catalytic mechanism of OAC, the enzyme was overexpressed in Escherichia coli and crystallized in a vapour-diffusion method. The crystals diffracted X-rays to 1.40 Å resolutions and belonged to space group P3121 or P3221, with unit-cell parameters a = b = 47.3 Å, c = 176.0 Å. Further crystallographic analysis will provide valuable insights into the structure–function relationship and catalytic mechanism of OAC. | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Acta Crystallogr F Struct Biol Commun, 2015 Dec; 71(Pt 12): 1470-4 | |||||
書誌情報 |
Acta Crystallographica Section F Structural Biology Communications 巻 71, 号 12, p. 1470-1474, 発行日 2015-12 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 17443091 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12097708 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1107/S2053230X15020385 | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
出版者 | ||||||
出版者 | International Union of Crystallography | |||||
資源タイプ(DSpace) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article |