@article{oai:toyama.repo.nii.ac.jp:00005346,
 author = {Komatsu, Katsuko and YANG, DongYe and Fushimi, Hirotoshi and CAI, ShaoQing},
 issue = {suppl.},
 journal = {和漢医薬学雑誌 = Journal of traditional medicines},
 month = {},
 note = {application/pdf, Rhei Rhizoma (Dahuang in Chinese) is widely known as a purgative and antiinflammatory agent. In the Japanese Pharmacopoeia, Rhei Rhizoma is prescribed for 4 Rheum species, Rheum palmatum, R. tanguticum, R. officinale, and R. coreanum, while the first 3 species are prescribed for Dahuang in the Chinese Pharmacopoeia. Due to the morphologic similarity of the aerial parts, the taxonomy of this genus and the correct identification of Rheum species and their derivative drugs are very difficult. To resolve taxonomic problems of the genus Rheum and develop an ultimate identification method for plants and drugs, molecular analysis of the chloroplast matK gene and nuclear 18S ribosomal RNA gene were performed on 9 species. The sequence comparison of the matK gene revealed that most species had variable sequences not only inter- but also intraspecies. However, the specimens of the same species belonged to the same subclade in the phylogenetic tree constructed based on matK gene sequences, except for R. palmatum, in which specimens belonged to 3 subclades related to their production areas. The nucleotide differences at positions 587,707, and 838 distinguished official species from others, while specific nucleotides at positions 367 and 937 became identification markers for R. palmatum, R. tanguticum, and R. officinale. Moreover, three groups of R. palmatum, each belonging to 3 subclades, were characterized by the nucleotides at positions 619,769,883, and 1061. On the basis of the above marker nucleotides, a convenient and efficient identification method employing Polymerase Chain Reaction-Restriction Fragment Length Polymorphism and Amplification Refractory Mutation System analyses was further developed. The procedure enabled us to identify the botanic origins of 22 drug samples of Rhei Rhizoma. The matK gene sequence was valuable in identifying Rheum species and Rhei Rhizoma and in predicting their production areas, and could be used as an index for quality evaluation of Rhei Rhizoma., Article},
 pages = {70--85},
 title = {Authentication of Rhei Rhizoma(Material study)},
 volume = {22},
 year = {2005}
}