@article{oai:toyama.repo.nii.ac.jp:00005335,
 author = {SHI, Liying and Tezuka, Yasuhiro and SUBE, HAN and Ueda, Jun-ya and Miyahara, Tatsuro and YIN, Jun and Nobukawa, Takahiro and Kadota, Shigetoshi},
 issue = {3},
 journal = {和漢医薬学雑誌 = Journal of traditional medicines},
 month = {Jul},
 note = {application/pdf, The inhibitory activities of 19 Chinese formulae on osteoclast formation and bone resorption in vitro were investigated. The inhibitory activity on osteoclast formation was evaluated using a mouse bone marrow cell culture in which osteoclast-like multinucleated cells were formed in the presence of parathyroid hormone (PTH). The inhibitory activity on bone resoption was evaluated using PTH-induced resorption of ^<45>Ca from ^<45>Ca-prelabeled neonatal mouse parietal bone. Among the 19 Chinese formulae, nine showed a strong inhibitory activity on osteoclast formation at a concentration of 50 μg/ml, and three of the nine also showed a strong inhibitory activity on bone resorption. Among these three formulae, Bu-Shen-Jian-Gu-Tang (補腎健骨湯) showed the most promising activity in a dose-dependent manner on the inhibition of bone resorption. A further in vivo experiment in ovariectomized (OVX) model rats using peripheral quantitative computed tomography (pQCT) revealed its preventive effect on bone loss.
本研究では19種の漢方方剤によるin vitroでの破骨細胞形成及び骨吸収の抑制活性を検討した。破骨細胞形成抑制活性はマウス骨髄細胞培養系を用い,副甲状腺ホルモン(PTH)存在下での破骨細胞様多核細胞の形成により評価した。骨吸収抑制活性は^<45>Ca標識した新生児マウスの頭頂骨での,PTH誘導による^<45>Ca吸収を評価した。19種の漢方方剤のうち,9処方が50μg/mlの濃度で強い破骨細胞形成抑制活性を示し,この中で,3処方は骨吸収を強く抑制した。中でも,「補腎健骨湯」は用量依存的に骨吸収を抑制し,最も有望な活性を示した。さらに,卵巣摘出(OVX)ラットを用いたin vivoでの実験では, pQCT(末梢骨定量的コンピューター断層撮影法)での評価の結果,骨減少を予防する効果が認められた。, Article},
 pages = {92--100},
 title = {Inhibitory effect of Kampo medicines on bone resorption in vitro and preventive effect on bone loss in vivo},
 volume = {23},
 year = {2006}
}