{"created":"2023-07-25T09:07:02.528328+00:00","id":5331,"links":{},"metadata":{"_buckets":{"deposit":"0574b5ca-ff6d-46dd-bca9-fdaf234742a4"},"_deposit":{"created_by":3,"id":"5331","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"5331"},"status":"published"},"_oai":{"id":"oai:toyama.repo.nii.ac.jp:00005331","sets":["646:647:648"]},"author_link":["159539","159544","159543","159540","159541","159542","159538"],"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"23","bibliographicPageStart":"19","bibliographicVolumeNumber":"24","bibliographic_titles":[{"bibliographic_title":"和漢医薬学雑誌 = Journal of traditional medicines"}]}]},"item_2_description_15":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The constituents of Bu-Shen-Jian-Gu-Tang and their inhibitory activities on osteoclast-Iike cell formation were investigated. Bu-Shen-Jian-Gu-Tang was fractionated by a Diaion HP-20 column with EtOH-H_2O, and the 60% and 90% EtOH-H_20 fractions showed more potent inhibitory activities on osteoclast-like cell formation and bone resorption. Chemical investigation of these two fractions resulted in the isolation of 11 compounds, for which the structures were elucidated based on spectroscopic analysis and chemical reactions. The inhibitory activities of the isolated compounds on osteoclast-like cell formation were evaluated. Among the eleven compounds, icariin (1), icarisid II (2), 2\"-O-rhamnosylicariside II (3), kaempferol (8), chikusetsusaponin IVa (10) and chikusetsusaponin V (11) showed potent inhibitory activities at a concentration of 2 μM without toxic effects. The preventive effect of Bu-Shen-Jian-Gu-Tang on bone loss may be due to the inhibitory activities of these compounds.\n 本研究では,補腎健骨湯について,Diaion HP-20カラムクロマトグラフィを用いて,H_2O,30%,60%及び90%EtOH-H_2Oに順次分画し,各フラクションの破骨細胞形成及び骨吸収の抑制活性を検討したところ,60%及び90%EtOH-H_2O溶出部に抑制活性が強いことが判った。さらに,これらのフラクションから単離した11種の化合物について,破骨細胞形成抑制活性を検討した。11種の化合物のうち,6種の化合物(icariin (1), icarisid II (2), 2\"-O-rhamnosylicariside II (3), kaempferol (8), chikusetsu-saponin IVa (10)およびchikusetsusaponin V (11))が補腎健骨湯の骨吸収抑制活性成分であることが明らかとなった。","subitem_description_type":"Abstract"}]},"item_2_description_40":{"attribute_name":"資源タイプ(DSpace)","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"159544","nameIdentifierScheme":"WEKO"}],"names":[{"name":"門田, 重利"}]}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"和漢医薬学会"}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"rights: 本文データは和漢医薬学会の許諾に基づきCiNiiから複製したものである"}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12035198","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"18801447","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tezuka, Yasuhiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miyahara, Tatsuro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"YIN, Jun"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nobukawa, Takahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kadota, Shigetoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SHI, Liying"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"detail","filename":"wakan07-19.pdf","filesize":[{"value":"576.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"wakan07-19.pdf","url":"https://toyama.repo.nii.ac.jp/record/5331/files/wakan07-19.pdf"},"version_id":"f64ec529-16e5-40bb-8572-589d7e73cbfd"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"antiosteoporotic activity","subitem_subject_scheme":"Other"},{"subitem_subject":"Kampo formula","subitem_subject_scheme":"Other"},{"subitem_subject":"Bu-Shen-Jian-Gu-Tang","subitem_subject_scheme":"Other"},{"subitem_subject":"osteoclast","subitem_subject_scheme":"Other"},{"subitem_subject":"inhibitory activity","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Inhibitory effect of constituents of Bu-Shen-Jian-Gu-Tang on osteoclast-like cell formation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Inhibitory effect of constituents of Bu-Shen-Jian-Gu-Tang on osteoclast-like cell formation"}]},"item_type_id":"2","owner":"3","path":["648"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-01-28"},"publish_date":"2008-01-28","publish_status":"0","recid":"5331","relation_version_is_last":true,"title":["Inhibitory effect of constituents of Bu-Shen-Jian-Gu-Tang on osteoclast-like cell formation"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-11-06T04:44:33.261625+00:00"}