@article{oai:toyama.repo.nii.ac.jp:00005304,
 author = {CHO, EunJu and Yokozawa, Takako and RHYU, DongYoung and Mitsuma, Tadamichi and Terasawa, Katsutoshi and PARK, JongCheol},
 issue = {6},
 journal = {和漢医薬学雑誌 = Journal of traditional medicines},
 month = {Dec},
 note = {application/pdf, We investigated Wen-Pi-Tang and its crude drug extracts to determine their protective effect from oxidative stress caused by the hydrophilic and lipophilic free radical generators, 2,2'-azobis (2-amidino-propane) dihydrochloride (AAPH) and 2,2'-azobis(2,4-dimethylvaleronitrile) (AMVN) in LLC-PK_1 renal tubular epithelial cells. In response to AAPH and AMVN treatment, cell viability decreased significantly and significantly enhanced thiobarbituric acid-reactive substances (TBARS) formation was observed. However, Wen-Pi-Tang and its crude drug extracts showed scavenging of peroxyl radicals, which were generated by AAPH and AMVN, resulting in greater cell viability and lower TBARS formation than controls treated only with free radical generators. In particular, Wen-Pi-Tang, Rhei Rhizoma and Ginseng Radix demonstrated high protective activity, whereas Aconiti Tuber, Zingiberis Rhizoma and Glycyrrhizae Radix showed relatively low activity. This result suggests that the antioxidant activity of Wen-Pi-Tang was attributable to the crude extracts, and that both act as hydrophilic and lipophilic antioxidants.
 水溶性アゾ化合物のAAPH(2,2'-azobis(2-amidino-propane)dihydrochloride)と脂溶性アゾ化合物のAMVN(2,2'-azobis(2,4dimethylvaleronitrile))で腎上皮細胞のLLC-PK1に酸化的ストレスを惹起させ,温脾湯と5種類の構成和漢薬の効果を検討した。AAPHとAMVNで処埋した場合,細胞生有率が著しく抵ドし,チオバルビツール酸反応物質の生成が著しく上昇したが,温脾湯と各構成和漢薬エキスをそれぞれ添加した場合,温脾湯と大黄,薬用人参では高い抗酸化活性を示した。しかし附子,乾姜,甘草では相対的に低いi活牲であった。このことから,温脾湯の抗酸化活性は構成和漢薬に起因し,また温脾湯は水溶性抗酸化物と脂溶性抗酸化物の両方の特徴を有していることが示唆された。, Article},
 pages = {245--252},
 title = {Protective activity from hydrophilic and lipophilic free radical generators of Wen-Pi-Tang and its crude drug extracts in LLC-PK_1 cells},
 volume = {17},
 year = {2000}
}