{"created":"2023-07-25T09:07:00.780220+00:00","id":5299,"links":{},"metadata":{"_buckets":{"deposit":"3d2c8fba-dc06-4b64-b3c7-b9fb72b2eb59"},"_deposit":{"created_by":3,"id":"5299","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"5299"},"status":"published"},"_oai":{"id":"oai:toyama.repo.nii.ac.jp:00005299","sets":["646:647:648"]},"author_link":["6030","159374","159375","159373"],"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2001-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"63","bibliographicPageStart":"58","bibliographicVolumeNumber":"18","bibliographic_titles":[{"bibliographic_title":"和漢医薬学雑誌 = Journal of traditional medicines"}]}]},"item_2_description_15":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"For the purpose of investigating the metabolic processes of shikonin (1) by human intestinal bacteria, we prepared a sonicated bacterial cell suspension and a crude enzyme preparation from Eubacterium sp. A-44, one of the intestinal bacteria capable of transforming 1 to various metabolites. After anaerobic incubation with the suspension for 1 hr, most of 1 was transformed to prometaboshi-konin (2), and metaboshikonins I (3) and II (4). However, under aerobic conditions, the dimers, shikometabolins A (5) and B (6), were predominantly formed. In the presence of the crude enzyme preparation, formation of 2-4 was inhibited by oxygen, but markedly enhanced by the addition of NADH. On the other hand, formation of 5 and 6 was appreciably accelerated by the addition of NAD^+. In the absence of the crude enzyme preparation, NADH and/or NAD^+ showed no ability to transform 1 to the any metabolites, as in the case of a thermally inactivated preparation. Accordingly, the two different metabolic processes leading to compounds 2-4 and com-pounds 5 and 6 by Eubacterium sp. A-44 are concluded to be enzyme-dependent in the presence of NADH and NAD^+.\n ヒト腸内細菌による紫根成分shikoninの代謝過程を研究する目的でshikonin(1)を種々の代謝物に変換する腸内細菌Eubacterium sp.A-44の細胞破砕懸濁液及び粗酵素標品を調製した。この粗酵素標品とshikonin(1)を嫌気条件下で1時間インキュベーションすると,shikonin(1)のほとんどはprometaboshikonin(2)およびmetaboshikonin I(3),II(4)に変換された。しかしながら,好気条件下ではshikometabolin A(5),B(6)などの二量体が生成した。化合物2-4の生成は,酸素によって阻害され,NADHの添加により増強した。一方,化合物5,6の生成はNAD^+の添加により増加した。加熱処理した場合と同様,粗酵素標品を除去した場合は,NADH,NAD^+を添加してもshikoninの変換は起こらなかった。したがって,NADHやNAD^+の存在下での化合物2-4や化合物5,6への変換過程は酵素反応によって触媒されることを示している。","subitem_description_type":"Abstract"}]},"item_2_description_40":{"attribute_name":"資源タイプ(DSpace)","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"和漢医薬学会"}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"rights: 本文データは和漢医薬学会の許諾に基づき複製したものである"}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN10486121","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13406302","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"MESELHY, MeselhyR."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nishimoto, Eri"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Akao, Teruaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hattori, Masao"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"detail","filename":"20010203.pdf","filesize":[{"value":"790.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"20010203.pdf","url":"https://toyama.repo.nii.ac.jp/record/5299/files/20010203.pdf"},"version_id":"0f05f796-9a30-4c90-9f51-fb3b6b39768f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Eubacterium","subitem_subject_scheme":"Other"},{"subitem_subject":"human intestinal bacteria","subitem_subject_scheme":"Other"},{"subitem_subject":"metabolism","subitem_subject_scheme":"Other"},{"subitem_subject":"prometaboshikonin","subitem_subject_scheme":"Other"},{"subitem_subject":"shikonin","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Transformation of shikonin by a cell-free extract of Eubacterium sp. A-44, a human intestinal bacterium","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Transformation of shikonin by a cell-free extract of Eubacterium sp. A-44, a human intestinal bacterium"}]},"item_type_id":"2","owner":"3","path":["648"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-01-24"},"publish_date":"2008-01-24","publish_status":"0","recid":"5299","relation_version_is_last":true,"title":["Transformation of shikonin by a cell-free extract of Eubacterium sp. A-44, a human intestinal bacterium"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-11-06T04:44:26.450836+00:00"}