@article{oai:toyama.repo.nii.ac.jp:00005243,
 author = {AGB, Eun-Mi and Akao, Teruaki and Nakamura, Norio and Komatsu, Katsuko and Nishimura, Tsutomu and Hattori, Masao},
 issue = {2},
 journal = {和漢医薬学雑誌 = Journal of traditional medicines},
 month = {Apr},
 note = {application/pdf, For the purpose of evaluating phytoestrogenic activity of medicinal plant extracts, a naringinase-pretreatment method was developed, monitoring with proliferation of MCF-7 human breast cancer cells and induction of β-galactosidase in a yeast two-hybrid assay system. 0f various medicinal plant extracts examined, the extracts of Alpinia katsumadai (seeds), Glycyrrhiza uralensis (roots) and Moghania philippinensis (roots) showed higher estrogenic activity by per-treatment with naringinase than the original extract themselves. The contents of liquiritigenin and isoliquiritigenin having potent estrogenic activity, appreciably increased after the naringinase treatment of the extract of G. uralensis. These findings suggested that orally administered crude drugs would increase their estrogenic activity, due to the hydrolysis of some glycosylated constituents by intestinal flora.
　薬用植物エキスの植物エストロゲン活性を評価する目的で,naringinaseで前処理し,MCF-7ヒト乳癌細胞の増植反びyeast two-hybrid assay法によるβ-galactosidase活性の誘導を指標とする方法を開発した。検討したエキスの中でALpinia katsumadai, Glycyrrhiza uralensis, Moghania philippinensisはnaringinase処理することにより,元のエキスよりエストロゲン活性が強くなった。G. uralensisのエキスをnaringinase処理すると,エストロゲン活性のあるliquiritigeninとisoliquiritigeninの含有量は顕著に増加した。この結果は,生薬を経口投与すると場内細菌による配糖体の加水分解が起り,エストロゲン活性が増大することを示唆している。, Article},
 pages = {81--86},
 title = {Screening of medicinal plant extracts for estrogenic activity in combination with a glycosidase treatment},
 volume = {21},
 year = {2004}
}