@article{oai:toyama.repo.nii.ac.jp:00005227,
 author = {Chung, MiHwa and Nakamura, Norio and Tohda, Michihisa and Hattori, Masao},
 issue = {1},
 journal = {和漢医薬学雑誌 = Journal of traditional medicines},
 month = {Feb},
 note = {application/pdf, 当帰芍薬散は古くから婦人科傾城で使われている代表漢方方剤の1つである。また,下垂体アデニル酸シクラーゼ活性化ポリペプテド(PACAP)は,視床下部において性腺刺激ホルモンの分泌促進,卵巣の順位膜細胞において女性ホルモン(エストラジオールとプロゲステロン)の生合成に重要な役割を持つことが知られている。しかしながら,当帰芍薬散のPACAPに対する影響は明らかにされていない。そこで我々は,下垂体摘出ラットと卵巣摘出ラットを用い,当帰芍薬散経口投与後の視床下部の解析を行った。両モデルラットにおいて,当帰芍薬散はPACAPのmRNA発現を促進した。またPACAP受容体(PAC_1)のmRNA,タンパク発現も促進した。しかしながら下垂体や卵巣の摘出により上昇した骨代謝を抑えることは出来なかった。以上の結果より,当帰芍薬散は視床下部におけるPACAP,PAC_1を経由して性腺刺激ホルモンの分泌促進を行っている可能性が示唆された。

The traditional Chinese medicine, Tokishakuyakusan (TS; Dang-Gui-Shao-Yao-San in Chinese), is frequently applied in obstetrics and gynecology departments in Japanese hospitals. The novel neuropeptide, pituitary adenylate cyclase-activating polypeptide (PACAP) that is transiently induced by the gonadotropin surge, plays an important role in the synthesis of estradiol and progesterone in ovarian granulosa cells. Here, we investigated the effect of TS on PACAP in hypophysectomized (HPX) or ovariectomized (OVX) female rats. The weight of the hypothalamus from HPX rats administered with oral TS for one week did not appreciably change. In contrast, TS decreased the weight of the hypothalamus that was increased by ovariectomy. Moreover, TS promoted the expression of PACAP and PACAP type I receptor (PAC_1) mRNA and protein in the hypothalamus of HPX and OVX rats. However, bone metabolism that was increased by removing the pituitary or ovaries was not suppressed. These findings suggest that TS promotes the secretion of gonadotropic hormone through the expression of hypothalamic PACAP, PAC_1 mRNA and PAC_1 protein., Article},
 pages = {24--30},
 title = {Effects of Tokishakuyakusan on hypothalamic pituitary adenylate cyclase-activating polypeptide (PACAP) and PACAP type I receptor (PAC_1) expression in hypophysectomized and ovariectomized rats},
 volume = {24},
 year = {2007}
}