@article{oai:toyama.repo.nii.ac.jp:00005226,
 author = {ZUO, Feng and HAN, Hao-feng and HIRAKAWA, Akiko and NAKAMURA, Norio and Hattori, Masao},
 issue = {1},
 journal = {和漢医薬学雑誌 = Journal of traditional medicines},
 month = {Feb},
 note = {application/pdf, 樟芝菌糸体中の新規マレイン酸及び琥珀酸誘導体(antrodin A-E)の定量分析法を確立する目的で,高速液体クロマトグラフィー(HPLC)による分析条件を検討した。Antrodin A-Eは,移動相:1%AcOH/H_2O-CH_3CN (0 min, 60: 40; 40min, 30:70);流速:1.0ml/min;測定波長:235nm;カラム温度:室温の条件下,ODSカラムを用い定量を行った。この方法による日内と日間の相対的な標準偏差は4.6%及び4.1%(n=5)であり,0.1 (or 0.2)μgから1.2μgまでの濃度範囲で良好な直線性が得られた。Antrodin A-Eの回収率はそれぞれ,99.8, 102.9, 101.0, 100.5及び99.9%であった。本測定法は迅速且つ正確であり,樟芝菌糸体中の新規マレイン酸及び琥珀酸誘導体の定量に適していると考えられる。
For the purpose of quantitative determination of novel maleic acid and succinic acid derivatives, antrodins A-E, in the mycelium of Antrodia cinnamomea, a high-performance liquid chromatography (HPLC) method was applied to separation of these compounds under the conditions, where the mobile phase was a linear gradient of 1% AcOH/H_2O-CH_3CN, the flow rate was 1.0 ml/min and the detecting wavelength was 235 nm, using an ODS column. The relative standard deviations of this method were less than 4.6% and 4.1% (n=5) for interday and intraday assays, respectively. A good linear correlation was obtained in a range of 0.1 (or 0.2) μg-1.2μg. The recoveries of antrodins A, B, C, D and E were 99.8, 102.9, 101.0, 100.5 and 99.9%, respectively. This method was rapid, accurate and suitable for the quantitative determination of maleic acid and succinic acid derivatives in the mycelium of Antrodia cinnamomea., Article},
 pages = {19--23},
 title = {Quantitative determination of maleic and succinic acid derivatives in the mycelium of Antrodia cinnamomea},
 volume = {23},
 year = {2006}
}