{"created":"2023-07-25T09:06:52.783037+00:00","id":5159,"links":{},"metadata":{"_buckets":{"deposit":"5ad16f79-aa1c-46aa-9166-dad7c380be5c"},"_deposit":{"created_by":3,"id":"5159","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"5159"},"status":"published"},"_oai":{"id":"oai:toyama.repo.nii.ac.jp:00005159","sets":["640:641:642"]},"author_link":["158858","158857"],"item_9_alternative_title_19":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"牛尾椎椎間板におけるインスリン様成長因子-1(IGF-1)の自己分泌機構及びIGF-1が椎間板のプロテオグリカン合成に与える影響"}]},"item_9_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1996-03-08","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{}]}]},"item_9_date_granted_64":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"1996-03-08"}]},"item_9_degree_grantor_62":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"富山医科薬科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"13202","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_9_degree_name_61":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)"}]},"item_9_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The present study was undertaken to investigate the effect of IGF-1 on proteoglycan (PG) synthesis and the autocrine/paracrine mechanisms involving insulin-like growth factor-1 (IGF-1) in the bovine coccygeal intervertebral disc.\nIGF-1 stimulated PG synthesis in cultured cells of nucleus pulposus of bovine intervertebral discs in a dose-dependent manner, and the effect was inhibited by an anti-IGF-1 monoclonal antibody.\nInsitu hybridization histochemistry revealed the expression of IGF-1 mRNA in the cultured cells, and IGF-1 production in these cells was demonstrated by radioimmunoassay.\nIGF-1 receptor in the cultured cells was also immunohistochemically demonstrated.\nThe Scatchard analysis using an ^<125>I-IGF-1 binding assay showed that the cells cultured in monolayer had a single type of IGF-1 receptor, whose affinity and number were estimated to be 7.38 x 10^8/M and 9.27 x 10^4/cell, respectively. These results suggest that IGF-1 stimulates PG synthesis in cells of the nucleus pulposus, and that these cells in culture have an IGF-1 autocrine/paracrine mechanism.\nThe expressions of IGF-1 mRNA and IGF-1 receptor in disc tissue were greater in cells of the nucleus pulposus of fetal bovine intervertebral discs than in those of the adult discs.\nThese findings suggest that the autocrine/paracrine IGF-1 action is more active in cells of young nucleus pulposus than in cells of matured subjects.","subitem_description_type":"Abstract"}]},"item_9_description_40":{"attribute_name":"資源タイプ(DSpace)","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"富山医科薬科大学・博士(医学)・甲第240号・長田龍介・1996/3/8","subitem_description_type":"Other"}]},"item_9_dissertation_number_65":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第240号"}]},"item_9_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"158858","nameIdentifierScheme":"WEKO"}],"names":[{"name":"長田, 龍介"}]}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.11501/3108972","subitem_identifier_reg_type":"JaLC"}]},"item_9_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Osada, Ryusuke"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"detail","filename":"000000293710.pdf","filesize":[{"value":"6.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"000000293710.pdf","url":"https://toyama.repo.nii.ac.jp/record/5159/files/000000293710.pdf"},"version_id":"0a385663-8598-4f4e-b634-4e88096eb481"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"insulin like growth factor-1","subitem_subject_scheme":"Other"},{"subitem_subject":"autocrine/paracrine mechanism","subitem_subject_scheme":"Other"},{"subitem_subject":"intervertebral disc","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Autocrine/Paracrine Mechanism of Insulin like Growth Factor-1 secretion, and the Effect of IGF-1 on Proteoglycan Synthesis in Bovine Intervertebral Discs","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Autocrine/Paracrine Mechanism of Insulin like Growth Factor-1 secretion, and the Effect of IGF-1 on Proteoglycan Synthesis in Bovine Intervertebral Discs"}]},"item_type_id":"9","owner":"3","path":["642"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-02-07"},"publish_date":"2013-02-07","publish_status":"0","recid":"5159","relation_version_is_last":true,"title":["Autocrine/Paracrine Mechanism of Insulin like Growth Factor-1 secretion, and the Effect of IGF-1 on Proteoglycan Synthesis in Bovine Intervertebral Discs"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-25T15:02:23.181070+00:00"}