@phdthesis{oai:toyama.repo.nii.ac.jp:00005144,
 author = {丸田, 喜美子},
 month = {Oct},
 note = {To examine the presence of substances which inhibit calcification in human ligamentum flavum, the calcification inhibitory effect of human ligamentum flavum extract obtained by extraction of the tissue with 3% Na_2HPO_4 was determined by the in vitro calcium uptake of the ligamentum flavum matrix.
In addition, grafts of extracted and non-extracted dry ligamentum flavum matrices were transplanted into the dorsal muscles of rats, and calcification in the grafts was examined radiologically and histochemically.
In order to determine whether or not component cells of human ligamentum flavum produce calcification inhibitors, ligamentum flavum cells were cultured, and thecrystal inhibitor activity of the culture medium was measured by the seed test using hydroxyapatite as the nucleus of precipitation.
The calcification reaction system demonstrated that the ligamentum flavum extract contains an inhibitory factor for calcium uptake by the ligamentum flavum matrix.
The seed test revealed that human ligamentum flavum cells produce calcification inhibitor activity.
The inhibitor activity was fractionated into two peaks by gel filtration of the culture medium., Article, 富山医科薬科大学・医学博士・乙第144号・丸田喜美子・1991/10/9},
 school = {富山医科薬科大学},
 title = {Calcification inhibitors in human ligamentum flavum},
 year = {1991}
}