{"created":"2023-07-25T09:06:16.601337+00:00","id":4506,"links":{},"metadata":{"_buckets":{"deposit":"966d41f2-1f36-4db9-81ce-e6ae04e0f9d0"},"_deposit":{"created_by":3,"id":"4506","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"4506"},"status":"published"},"_oai":{"id":"oai:toyama.repo.nii.ac.jp:00004506","sets":["496:556:559:560:606"]},"author_link":["57893","15877","57889","57891","57895","57896","57892"],"item_3_alternative_title_19":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Production and Characterization of Ligninolytic Enzymes by Coriolus versicolor"}]},"item_3_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1995-02","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"116","bibliographicPageStart":"109","bibliographicVolumeNumber":"46","bibliographic_titles":[{"bibliographic_title":"富山大学工学部紀要"}]}]},"item_3_description_15":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"It has been reported that C. versicolor produces extracellarly lignin-decomposing enzymes such as laccase and lignin peroxidase. In order to achieve the high yield of two enzymes, we cultured the fungi by the surface culture method, investigated the production of enzymes.\nMoreover, purification and characterization of these enzymes were also investigated. As a result, the activities of laccase and lignin peroxidase achieved about 1.60, and 0.45nkat/ml, respectively. Further, it was found that two enzymes could be purified easily by DEAE-Toyopearl and the molecular weights of laccase and lignin peroxidase were estimated to be 38,000, 42,000, using electrophpresis, respectively.","subitem_description_type":"Abstract"},{"subitem_description":"白色腐朽菌C.versicolor IFO 4937は，木材中のリグニンを分解するラッカーゼやリグニンパーオキシダーゼを生産すると報告されている。本研究では，より効率的な酵素生産を目的として，表面培養法を用いたリグニン分解酵素の生産について検討した。さらに，この2種の酵素を分離精製し，それぞれの性質も検討した。その結果，本菌株は最大酵素活性でラッカーゼ，およびリグニンパーオキシダーゼを1.60，および0.45nkat/ml生産することができた。また，両酵素はDEAE-Toyopearlを用いたイオン交換クロマトグラフィーにより容易に分離精製できた。精製したラッカーゼ，リグニンパーオキシダーゼの分子量はポリアクリルアミドゲル電気泳動によりそれぞれ38,000，42,000であることがわかった。","subitem_description_type":"Abstract"}]},"item_3_description_40":{"attribute_name":"資源タイプ(DSpace)","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_3_description_6":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"富山大学工学部紀要，46, Page 109-116","subitem_description_type":"Other"}]},"item_3_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"57893","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Kawaguchi, KaKouichi"}]},{"nameIdentifiers":[{"nameIdentifier":"15877","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000002438896","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000002438896"},{"nameIdentifier":"20222276","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000020222276"}],"names":[{"name":"Hoshino, Kazuhiro"}]},{"nameIdentifiers":[{"nameIdentifier":"57895","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Morohashi, Shoichi"}]},{"nameIdentifiers":[{"nameIdentifier":"57896","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Sasakura, Toshisuke"}]}]},"item_3_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15099/00004500","subitem_identifier_reg_type":"JaLC"}]},"item_3_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"富山大学工学部"}]},"item_3_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00175872","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03871339","subitem_source_identifier_type":"ISSN"}]},"item_3_subject_22":{"attribute_name":"国立国会図書館分類","attribute_value_mlt":[{"subitem_subject":"ZM2","subitem_subject_scheme":"NDLC"}]},"item_3_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"川口, 浩一"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"星野, 一宏"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"諸橋, 昭一"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"笹倉, 寿介"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-16"}],"displaytype":"detail","filename":"Kokiyo_46_01_Page109to116.pdf","filesize":[{"value":"5.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Kokiyo_46_01_Page109to116.pdf","url":"https://toyama.repo.nii.ac.jp/record/4506/files/Kokiyo_46_01_Page109to116.pdf"},"version_id":"4fa474d5-6fc5-44fa-be28-a6aa014f6844"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Coriolus versicolor","subitem_subject_scheme":"Other"},{"subitem_subject":"リグニン分解酵素","subitem_subject_scheme":"Other"},{"subitem_subject":"酵素","subitem_subject_scheme":"Other"},{"subitem_subject":"担子菌","subitem_subject_scheme":"Other"},{"subitem_subject":"キノコ","subitem_subject_scheme":"Other"},{"subitem_subject":"リグニンの有効利用","subitem_subject_scheme":"Other"},{"subitem_subject":"リグニン分解関連酵素","subitem_subject_scheme":"Other"},{"subitem_subject":"白色腐朽菌","subitem_subject_scheme":"Other"},{"subitem_subject":"リグニン分解","subitem_subject_scheme":"Other"},{"subitem_subject":"イオン交換クロマトグラフィー","subitem_subject_scheme":"Other"},{"subitem_subject":"ポリアクリルアミドゲル電気泳動","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Coriolus versicolorによるリグニン分解酵素の生産および酵素の性質","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Coriolus versicolorによるリグニン分解酵素の生産および酵素の性質"}]},"item_type_id":"3","owner":"3","path":["606"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-10-15"},"publish_date":"2012-10-15","publish_status":"0","recid":"4506","relation_version_is_last":true,"title":["Coriolus versicolorによるリグニン分解酵素の生産および酵素の性質"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-25T13:58:21.296136+00:00"}