@article{oai:toyama.repo.nii.ac.jp:00002627,
 author = {MATSUBARA1, Hisayo and YOSHII, Miho and MIYAHARA, Tatsuro and OBI, Nobuko and OCHIAI, Hiroshi},
 issue = {1},
 journal = {富山大学看護学会誌},
 month = {Sep},
 note = {We investigated the infection-preventive characteristics of Japanese green tea (middle grade) in reference to itscatechin (CAT) concentration. Colorimetric analysis showed that CAT was extracted most effectively at 80℃. When green tea extract (GTE) was prepared from the same green tea leaves (5g/80ml water) repeatedly six times at 80℃ for 1 min, almost the 2/3 of total CAT content was extracted during the first three extractions in which the second extraction gave the highest CAT concentration. Therefore, GTE prepared under this condition (5g/80ml water at 80℃ for 1 min) was referred to as standard GTE (SGTE) in this study. Bacteriological studies using Staphylococcus aureus, Escherichiac oli and Pseudomonas aeruginosa showed that the maximum inhibitory and bactericidal dilutions of the second SGET were ranged in 8-and4-fold dilutions, corresponding to 0.33mg/ml and 0.68mg/ml CAT concentrations, respectively, against either bacterial species and the differences between these two values were within 2-fold dilutions, indicating that SGTE shows the bactericidal activity. However, SGTE required as long as 4 to 5 h for bactericidal time in sharp contrast to iodine with that of as short as 1 min. When SGTE with CAT concentration ranging 1.2 mg/ml to 2.7mg/ml was incubated with influenza A/Aichi virus at 37℃ for at least 30min, SGTE also completely inhibited viral hemagglutination (HA) activity essential for the viral attachment to cell surface. The CAT-deficient SGTE, prepared by the treatment with FeCl_3, could not show both bactericidal and HA inhibitory activities, confirming that CAT in GTE is mainly responsible for these activities. In summary, GTE might be taken as a beneficial herbal medicine to promote the infection-safety conditions in the patients. However, on the application of GTE, its advantage/disadvantage and presence of sufficient CAT concentration should betightly recognized., 緑茶（煎茶）の抗菌性とインフルエンザウイルス赤血球凝集（HA）活性阻害の特性を検証した．酒石酸鉄比色法による検討から，低温域（50℃と65℃）より高温域（80℃と91℃）でより多くカテキン（CAT）は抽出され，就中80℃が最大CAT量を示した．5g茶葉/80ml水，80℃，1分（標準緑茶液:SGTE）で６回抽出したところ，２回目で最高CAT量（2.7mg/ml）を示し，３回目までにほぼその2/3が抽出された．黄色ブドウ球菌，大腸菌と緑膿菌に対するSGTEの最大発育阻止と最大殺菌希釈度の差は，どの菌に対しても２倍以下であり，緑茶は，広範囲性かつ殺菌的作用を示すことが確認された．但し，ヨード液の１分に比べ，殺菌時間は４～５時間と極めて長いことが注目された．種々CAT量を有するSGTEを，種々時間37℃でインフルエンザウイルスと接触させたところ，CAT量1.2mg/ml～2.7mg/mlの範囲で，30分接触で完全にHA活性を阻害した．さらに，塩化第二鉄処理によるCAT除去SGTEには，抗菌作用も抗HA作用もないことから，種々ある緑茶成分のうち，CATが主な有効成分であることが示された．これらから，緑茶を感染予防として活用する際には，遅効性であること，また十分量なCAT含有（少なくとも1mg/ml）ことの認識は重要であると思われた．, Article, 富山大学看護学会誌, 第7巻1号, 2007.9., page 23-31},
 pages = {23--31},
 title = {Study on the infection-preventive characteristics of Japanese green tea},
 volume = {7},
 year = {2007}
}