{"created":"2023-07-25T09:04:43.386835+00:00","id":2596,"links":{},"metadata":{"_buckets":{"deposit":"7101653a-a735-4491-9467-0fa438ba2778"},"_deposit":{"created_by":3,"id":"2596","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2596"},"status":"published"},"_oai":{"id":"oai:toyama.repo.nii.ac.jp:00002596","sets":["439:440:443:444:452"]},"author_link":["156575","156577","156581","156580","156578","156576","156574","156573","156572","156579"],"item_3_alternative_title_19":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Comparative study of genotyping methods for tracking the sources and routes of Serratia marscens, a representative environmental bacteria, for introduction to the infection control nurses"}]},"item_3_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"44","bibliographicPageStart":"27","bibliographicVolumeNumber":"6","bibliographic_titles":[{"bibliographic_title":"富山医科薬科大学看護学会誌"}]}]},"item_3_description_15":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"環境汚染菌による病院感染において,その感染に関わった最終的な汚染器具や医療行為は特定された例があるが,それに至る汚染源や伝播経路が明らかにされた例は極めて少ない.このようなことは病院感染対策上重要であり,感染管理看護師(ICN)も病院感染対策チームの一員として協力することが期待される.そこで,環境汚染菌の代表的存在であるセラチア菌16菌株を対象として伝播経路追求法として有用なRandom amplified polymorphic DNA polymerase chain reaction(RAPD-PCR)法,Pulsed field gel electrophoresis (PFGE)法とInfrequent restriction site PCR (IRS-PCR)法の3つの方法を,看護分野への導入の視点から,主として簡便性と識別精度の点から比較検討した.その結果,供試16菌株は,最多で15タイプ(RAPD-PCR法,プライマー1),最少で7タイプ(RAPD-PCR法,プライマー3)に識別された.RAPD-PCR(プライマー1)とPFGE法(13タイプ)の間で識別精度では大きな差は無かったものの,検査に要する時間では両法間に大きな差があった(前者2日と後者7日).また,手技の簡便性でも,制限酵素処理や複雑な電気泳動を必要としない点で前者に有利性が認められた.これらのことから,少なくともセラチア菌による病院感染流行発生時,その対策構築に協力するICNの立場から,汚染源・伝播経路追求をとしてRAPD-PCR(プライマー1)を採用することが推奨された.","subitem_description_type":"Abstract"},{"subitem_description":"To search the most convenient genotyping methods for the infection control nurses (ICN), three methods were compared from the aspects of time- and money-consumption, and methodological simplicity in addition to strain-differenciating efficiency using 16 strains of Serratia marscens, a representative environmental bacteria. Among the three methods, random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) using three different primers (primers 1 to 3 ), pulsed field gel electrophoresis (PFGE) and infrequent restriction site PCR (IRS-PCR) methods, 16 strains were distinguished from 15 types in PAPD-PCR ( primer 1) to 7 types in PAPD-PCR (primer 3) . In contrast to a small different in typing efficiency between PAPD-PCR ( primer 1) and PFGE (13 types), there were a large different in time-consumption ( 2 days in the former versus 7 days in the latter). Furthermore, PAPD-PCR had a merit in methodological simplicity such as carrying out without restriction enzyme treatment and complex gel electrophresis in PFGE which might be also reflected to money-consumption. In light of these findings, at least we can say that PAPD-PCR ( primer 1) is recommended to the ICN in an attemption for tracking of sources and routes of outbreak of Serratia marscens infections in the health care facilities.","subitem_description_type":"Abstract"}]},"item_3_description_40":{"attribute_name":"資源タイプ(DSpace)","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_3_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"156577","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Ueda, Kyoka"}]},{"nameIdentifiers":[{"nameIdentifier":"156578","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Sato, Miyuki"}]},{"nameIdentifiers":[{"nameIdentifier":"156579","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Katada, Yuko"}]},{"nameIdentifiers":[{"nameIdentifier":"156580","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Imanishi, Nobuko"}]},{"nameIdentifiers":[{"nameIdentifier":"156581","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Ochiai, Hiroshi"}]}]},"item_3_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15099/00002590","subitem_identifier_reg_type":"JaLC"}]},"item_3_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"富山医科薬科大学看護学会"}]},"item_3_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11340152","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1344-1434","subitem_source_identifier_type":"ISSN"}]},"item_3_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"上田, 京佳"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"佐藤, 美友紀"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"片田, 裕子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"今西, 信子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"落合, 宏"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-15"}],"displaytype":"detail","filename":"jns060104.pdf","filesize":[{"value":"3.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"jns060104.pdf","url":"https://toyama.repo.nii.ac.jp/record/2596/files/jns060104.pdf"},"version_id":"f8f42b86-c8da-47e5-8486-fa88b48dde54"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"セラチア菌","subitem_subject_scheme":"Other"},{"subitem_subject":"分子疫学","subitem_subject_scheme":"Other"},{"subitem_subject":"環境汚染","subitem_subject_scheme":"Other"},{"subitem_subject":"感染看護","subitem_subject_scheme":"Other"},{"subitem_subject":"Serratia marscens","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"分子疫学的手法によるセラチア菌の識別とその精度に関する研究 : ICNへの導入の視点から","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"分子疫学的手法によるセラチア菌の識別とその精度に関する研究 : ICNへの導入の視点から"}]},"item_type_id":"3","owner":"3","path":["452"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-12-21"},"publish_date":"2007-12-21","publish_status":"0","recid":"2596","relation_version_is_last":true,"title":["分子疫学的手法によるセラチア菌の識別とその精度に関する研究 : ICNへの導入の視点から"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-25T15:55:56.713443+00:00"}