{"created":"2023-07-25T09:04:39.009473+00:00","id":2523,"links":{},"metadata":{"_buckets":{"deposit":"b4966d41-ed73-43af-bc6e-33391b931d0e"},"_deposit":{"created_by":3,"id":"2523","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2523"},"status":"published"},"_oai":{"id":"oai:toyama.repo.nii.ac.jp:00002523","sets":["439:440:443:444:446"]},"author_link":["156126","156127","156125","156128","156130","156132","156129","156131"],"item_3_alternative_title_19":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Comparative studies on the strain-distinguishing efficiency among drug sensitivity tests, dru genes products and restriction flagment length polymorphism (RFLP) -typing of methcillin- resistant Staphylococcus aureus (MRSA)"}]},"item_3_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999-03","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"160","bibliographicPageStart":"151","bibliographicVolumeNumber":"2","bibliographic_titles":[{"bibliographic_title":"富山医科薬科大学看護学会誌"}]}]},"item_3_description_15":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"臨床材料由来メチシリン耐性黄色ブドウ球菌(MRSA)20株メチシリン感受性黄色ブドウ球菌(MSSA)1株の計21株を用い, 16剤の薬剤感受性試験を行ったところ, MSSAとは明らかに異なり, MRSAは全株とも12剤に耐性, また2剤(ST合剤, バイコマイシン)に感受性であった.しかし, ゲンタマイシンとミノマイシンに対する感受性は株により異なり, この2剤の感受性パターンに基づいて4タイプに分類された.polymerase chain reaction(PCR)によりmecAおよびdru遺伝子を増幅したところ, これらの遺伝子はMRSA株にのみ検出され, MRSAの同定に有用であることが確認できた.特にdru遺伝子増幅産物の塩基長は株依存性変異を示し, 404bpと501bpの2タイプに分類された.パルスフィールド電気泳動法で検出された染色体DNAのrestriction fragment length polymorphism(RFLP)からは13タイプに分類された.また, 薬剤感受性タイプのある群とdru塩基長タイプのある群には, それぞれ7つの異なるRFLPタイプが分布したことから, 薬剤感受性あるいはdru塩基長ダイビングの菌株識別精度は, RFLPダイビングの少なくとも1/7であることが示唆された.菌株の由来病室を指標にしてみたところ, 同一RFLPタイプに属した菌株群の一部は特定病室から集中的に分離されており, そこでの交差感染の存在を推定することができた.これらの結果から, 感染源・伝播経路追求にRFLP分析は極めて有効な分子疫学的手法であると考えられた.","subitem_description_type":"Abstract"},{"subitem_description":"To seach the most indicative epidemiological marker of methcillin-resistant Staphy-lococcus aureus (MRSA), we compared the strain-distinguishing effisiency among several typing methods such as drug-sensitivity test, analysis of polymerase chain reaction (PCR) -amplified products of mecA and dru genes, and restriction flagment length polymorphism (RFLP) -typing after digestion of chromosomal DNA with restriction enzyme of Sma I, using 20 MRSA strains isolated from the hospitalized patients in various wards. Drug sensitivity tests against 16 drugs showed that all MRSA strains were equally resistant or sensitive to 12 drugs or two drugs (vancomycin and sulfamethoxazole/trimethoprim), respectively, but these strains could be divided into 4 types depending on the different sensitivity profiles in only the remaining two drugs (gentamycin and minomycin). PCR assay confirmed that all MRSA strains possess both mecA and dru genes, whereas a control strain of methcillin-sensitive Staphylococcus aureus does not. Although mecA gene was amplified into single product with 531 base pair (bp) in all strains, amplified product of dru gene drew a dividing product into two bands either with 404 or 501 bp depending on the strains. In a sharp contrast to a fewer types in drug-sensitivity and dru typings, RFLP-typing could classified these strains into as much as 13 types, suggesting that RFLP-typing could provide the most indicative epidemiological information among the typings examined in this study. In a mimic epidemiological study, an occurrence of cross-infection was estimated in one patient room at which a cluster of strains with the same marker in RFLP was isolated within a relatively short period. These findings indicate that RFLP is a powerful tool for search the source of infections and desseminating routes to prevent nosocomial infections with MRSA","subitem_description_type":"Abstract"}]},"item_3_description_40":{"attribute_name":"資源タイプ(DSpace)","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_3_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"156129","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Umatake, Miho"}]},{"nameIdentifiers":[{"nameIdentifier":"156130","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Ochiai, Hiroshi"}]},{"nameIdentifiers":[{"nameIdentifier":"156131","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Yoshida, Ikuko"}]},{"nameIdentifiers":[{"nameIdentifier":"156132","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Tazawa, Kenji"}]}]},"item_3_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15099/00002517","subitem_identifier_reg_type":"JaLC"}]},"item_3_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"富山医科薬科大学看護学会"}]},"item_3_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11340152","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1344-1434","subitem_source_identifier_type":"ISSN"}]},"item_3_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"馬竹, 美穂"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"落合, 宏"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"吉田, 郁子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"田澤, 賢次"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-15"}],"displaytype":"detail","filename":"jns020016.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"jns020016.pdf","url":"https://toyama.repo.nii.ac.jp/record/2523/files/jns020016.pdf"},"version_id":"2ef3d022-753c-4929-82de-70ff83c0f7a9"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"MRSA(メチシリン耐性黄色ブドウ球菌)","subitem_subject_scheme":"Other"},{"subitem_subject":"RFLP","subitem_subject_scheme":"Other"},{"subitem_subject":"dru","subitem_subject_scheme":"Other"},{"subitem_subject":"院内感染","subitem_subject_scheme":"Other"},{"subitem_subject":"MRSA (methcillin-resistant Staphylococcus aureus)","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"薬剤感受性，dru塩基長および制限酵素断片分析のMRSA菌株識別精度に関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"薬剤感受性，dru塩基長および制限酵素断片分析のMRSA菌株識別精度に関する研究"}]},"item_type_id":"3","owner":"3","path":["446"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-12-21"},"publish_date":"2007-12-21","publish_status":"0","recid":"2523","relation_version_is_last":true,"title":["薬剤感受性，dru塩基長および制限酵素断片分析のMRSA菌株識別精度に関する研究"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-25T15:54:38.418523+00:00"}