{"created":"2023-07-25T09:04:25.534738+00:00","id":2261,"links":{},"metadata":{"_buckets":{"deposit":"5c7fa546-bbc0-4594-811e-a34d2774f78c"},"_deposit":{"created_by":3,"id":"2261","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2261"},"status":"published"},"_oai":{"id":"oai:toyama.repo.nii.ac.jp:00002261","sets":["439:440:441:442"]},"author_link":["153949","153951","153952","153950","153954","153953"],"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1995-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"10","bibliographicPageEnd":"727","bibliographicPageStart":"723","bibliographicVolumeNumber":"35","bibliographic_titles":[{"bibliographic_title":"Neurologia medico-chirurgica : 神経外科"}]}]},"item_2_description_15":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The effects of specific antibodies against growth factors and receptors on deoxyribonucleic acid (DNA) synthesis in two established human glioma cell lines, A172 and TM-1, were examined. Anti-platelet-derived growth factor (PDGF), anti-basic fibroblast growth factor (bFGF), and anti-epidermal growth factor receptor (EGF-R) antibodies inhibited thymidine incorporation by both cell lines in serum-free medium. Antibody specific to transforming growth factor-α only slightly suppressed DNA synthesis by both cell lines. Although the antiproliferative effects of anti-PDGF and anti-bFGF antibodies decreased in serum-supplemented medium, the effect of anti-EGF-R antibody was little changed. The combination of anti-PDGF, anti-bFGF, and anti-EGF-R antibodies significantly inhibited thymidine incorporation by the two cell lines even in serum-supplemented medium. This preliminary study suggests that simultaneous blockade of multiple autocrine loops may provide a new approach to the treatment of human malignant gliomas.\n 成長因子と成長因子受容体に対する特異的抗体を用いて、ヒト培養グリオーマ細胞2株(A172, TM-1)の増殖抑制作用を検討した。抗PDGF、抗bFGF、抗EGF-Rは、単独で両グリオーマ細胞の無血清培地中におけるDNA合成を抑制した。これら抗体のDNA合成抑制作用は、前2者では血清添加培地中では減弱したが、抗EGF-Rの作用は変わらなかった。抗TGF-αのDNA合成抑制作用は弱く、血清添加培地中ではその作用は認められなかった。抗PDGF、抗bFGF、抗EGF-Rの3抗体を同時に作用させると両細胞のDNA合成は、血清添加培地中においても著しく抑制された。複数のオートクリンループを遮断することは、培養グリオーマ細胞の増殖抑制には有意義な手段であると考えられた。","subitem_description_type":"Abstract"}]},"item_2_description_40":{"attribute_name":"資源タイプ(DSpace)","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"153954","nameIdentifierScheme":"WEKO"}],"names":[{"name":"栗本, 昌紀"}]}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本脳神経外科学会"}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"rights: 本文データは日本脳神経外科学会の許諾に基づきCiNiiから複製したものである"}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00358613","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"4708105","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kurimoto, Masanori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nogami, Kanehito"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ogiichi, Tsuneaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nishijima, Michiharu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takaku, Akira"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-15"}],"displaytype":"detail","filename":"shinkeigeka95-723.pdf","filesize":[{"value":"468.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"shinkeigeka95-723.pdf","url":"https://toyama.repo.nii.ac.jp/record/2261/files/shinkeigeka95-723.pdf"},"version_id":"4dceaefd-2e2d-41e4-911b-61709d6a819e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"multiple autocrine loops","subitem_subject_scheme":"Other"},{"subitem_subject":"platelet-derived growth factor","subitem_subject_scheme":"Other"},{"subitem_subject":"basic fibroblast growth factor","subitem_subject_scheme":"Other"},{"subitem_subject":"epidermal growth factor receptor","subitem_subject_scheme":"Other"},{"subitem_subject":"transforming growth factor-α","subitem_subject_scheme":"Other"},{"subitem_subject":"glioma cells","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Antiproliferative Effect of Multiple Autocrine Loop Blockade in Human Malignant Glioma Cell Lines","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Antiproliferative Effect of Multiple Autocrine Loop Blockade in Human Malignant Glioma Cell Lines"}]},"item_type_id":"2","owner":"3","path":["442"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-01-31"},"publish_date":"2008-01-31","publish_status":"0","recid":"2261","relation_version_is_last":true,"title":["Antiproliferative Effect of Multiple Autocrine Loop Blockade in Human Malignant Glioma Cell Lines"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-25T16:17:25.297097+00:00"}