{"created":"2023-07-25T09:15:46.647210+00:00","id":15770,"links":{},"metadata":{"_buckets":{"deposit":"50b74dfa-9fee-49af-b9ec-a2e8985fa1b0"},"_deposit":{"created_by":19,"id":"15770","owners":[19],"pid":{"revision_id":0,"type":"depid","value":"15770"},"status":"published"},"_oai":{"id":"oai:toyama.repo.nii.ac.jp:00015770","sets":["496:556:557:558"]},"author_link":["35378","35920","167916","30046","9692","167917","39523"],"item_2_alternative_title_19":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Evaluation of Amino Acid-Mustard Transport as L-Type Amino Acid Transporter 1 (LAT1)-Mediated Alkylating Agents"}]},"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-11-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageEnd":"2130","bibliographicPageStart":"2126","bibliographicVolumeNumber":"31","bibliographic_titles":[{"bibliographic_title":"Biological and Pharmaceutical Bulletin"},{"bibliographic_title":"Biological and Pharmaceutical Bulletin"}]}]},"item_2_description_15":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The L-type amino acid transporter 1 (LAT1, SLC7A5) is an Na+-independent neutral amino acid transporter the expression of which is located in retinal endothelial cells. Due to its broad substrate selectivity, LAT1 has been proposed to mediate the transport of amino acid-related drugs across the blood-tissue barriers. Here, we have investigated the transport screening of amino acid-mustards using a conditionally immortalized rat retinal capillary endothelial cell line (TR-iBRB2) which expresses LAT1. We synthesized 5 amino acid-mustards: tyrosine-mustard, phenylglycine-mustard, alanine-mustard, ornithine-mustard, and lysine-mustard. LAT1-mediated [3H]L-phenylalanine (Phe) uptake by TR-iBRB2 cells was inhibited in a competitive manner by tyrosine-mustard and phenylglycine-mustard as well as melphalan (phenylalanine-mustard). Phenylglycine-mustard was able to induce the efflux of [3H]Phe preloaded into the TR-iBRB2 cells expressing LAT1 through the obligatory exchange mechanism, although tyrosine-mustard, alanine-mustard, ornithine-mustard, lysine-mustard, and melphalan did not induce any significant efflux. These findings suggest that phenylglycine-mustard is a better substrate for LAT1 than melphalan and other amino acid-mustards.","subitem_description_type":"Abstract"}]},"item_2_description_40":{"attribute_name":"資源タイプ(DSpace)","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_description_6":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Biological and Pharmaceutical Bulletin 31(11), 2126-2130.(2008)","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"THE PHARMACEUTICAL SOCIETY OF JAPAN"},{"subitem_publisher":"公益社団法人日本薬学会"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1248/bpb.31.2126","subitem_relation_type_select":"DOI"}}]},"item_2_relation_29":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"110006968099","subitem_relation_type_select":"NAID"}}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10885497","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09186158","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hosoya, Ken-ichi"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Kyoko, Hirokazu"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Toyooka, Naoki"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Kato, Atsushi"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Orihashi, Masahiro"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Tomi, Masatoshi"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Tachikawa, Masanori"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-03-01"}],"displaytype":"detail","filename":"BiolPharmBull_31_11_2126to2130_Toyooka.pdf","filesize":[{"value":"200.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"BiolPharmBull_31_11_2126to2130_Toyooka","url":"https://toyama.repo.nii.ac.jp/record/15770/files/BiolPharmBull_31_11_2126to2130_Toyooka.pdf"},"version_id":"160d87ed-c8be-4297-a8ef-97c320ab2ae9"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"L-type amino acid transporter 1","subitem_subject_scheme":"Other"},{"subitem_subject":"amino acid-mustard","subitem_subject_scheme":"Other"},{"subitem_subject":"alkylating agent","subitem_subject_scheme":"Other"},{"subitem_subject":"transport","subitem_subject_scheme":"Other"},{"subitem_subject":"inner blood-retinal barrier","subitem_subject_scheme":"Other"},{"subitem_subject":"L-type amino acid transporter 1","subitem_subject_scheme":"Other"},{"subitem_subject":"amino acid-mustard","subitem_subject_scheme":"Other"},{"subitem_subject":"alkylating agent","subitem_subject_scheme":"Other"},{"subitem_subject":"transport","subitem_subject_scheme":"Other"},{"subitem_subject":"inner blood-retinal barrier","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Evaluation of Amino Acid-Mustard Transport as L-Type Amino Acid Transporter 1 (LAT1)-Mediated Alkylating Agents","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Evaluation of Amino Acid-Mustard Transport as L-Type Amino Acid Transporter 1 (LAT1)-Mediated Alkylating Agents"},{"subitem_title":"Evaluation of Amino Acid-Mustard Transport as L-Type Amino Acid Transporter 1 (LAT1)-Mediated Alkylating Agents","subitem_title_language":"en"}]},"item_type_id":"2","owner":"19","path":["558"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-03-01"},"publish_date":"2018-03-01","publish_status":"0","recid":"15770","relation_version_is_last":true,"title":["Evaluation of Amino Acid-Mustard Transport as L-Type Amino Acid Transporter 1 (LAT1)-Mediated Alkylating Agents"],"weko_creator_id":"19","weko_shared_id":-1},"updated":"2023-07-25T14:23:28.538520+00:00"}