{"created":"2023-07-25T09:15:44.249947+00:00","id":15714,"links":{},"metadata":{"_buckets":{"deposit":"4fe9f06f-8206-4c06-a465-4f0b8d4b2635"},"_deposit":{"created_by":19,"id":"15714","owners":[19],"pid":{"revision_id":0,"type":"depid","value":"15714"},"status":"published"},"_oai":{"id":"oai:toyama.repo.nii.ac.jp:00015714","sets":["496:556:557:558"]},"author_link":["167768","167765","167762","167763","167767","167770","167750","167769","167772","167754","167761","167764","167760","167771","167766"],"item_2_alternative_title_19":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Phospholipase Cbeta4 and protein kinase Calpha and/or protein kinase CbetaI are involved in the induction of long term depression in cerebellar Purkinje cells."}]},"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2001-11-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"48","bibliographicPageEnd":"45242","bibliographicPageStart":"45236","bibliographicVolumeNumber":"276","bibliographic_titles":[{"bibliographic_title":"The Journal of biological chemistry"},{"bibliographic_title":"The Journal of biological chemistry"}]}]},"item_2_description_15":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"Application/pdf","subitem_description_type":"Other"}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Activation of the type-1 metabotropic glutamate receptor (mGluR1) signaling pathway in the cerebellum involves activation of phospholipase C (PLC) and protein kinase C (PKC) for the induction of cerebellar long term depression (LTD). The PLC and PKC isoforms that are involved in LTD remain unclear, however. One previous study found no change in LTD in PKCgamma-deficient mice, thus, in the present study, we examined cerebellar LTD in PLCbeta4-deficient mice. Immunohistochemical and Western blot analyses of cerebellum from wild-type mice revealed that PLCbeta1 was expressed weakly and uniformly, PLCbeta2 was not detected, PLCbeta3 was expressed predominantly in caudal cerebellum (lobes 7-10), and PLCbeta4 was expressed uniformly throughout. In PLCbeta4-deficient mice, expression of total PLCbeta, the mGluR1-mediated Ca(2+) response, and LTD induction were greatly reduced in rostral cerebellum (lobes 1-6). Furthermore, we used immunohistochemistry to localize PKCalpha, -betaI, -betaII, and -gamma in mouse cerebellar Purkinje cells during LTD induction. Both PKCalpha and PKCbetaI were found to be translocated to the plasmamembrane under these conditions. Taken together, these results suggest that mGluR1-mediated activation of PLCbeta4 in rostral cerebellar Purkinje cells induced LTD via PKCalpha and/or PKCbetaI.","subitem_description_type":"Abstract"}]},"item_2_description_40":{"attribute_name":"資源タイプ(DSpace)","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_description_6":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"The Journal of biological chemistry 276(48), 45236-45242.(2001)","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Society for Biochemistry and Molecular Biology"}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"11551922","subitem_relation_type_select":"PMID"}}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1074/jbc.M105413200","subitem_relation_type_select":"DOI"}}]},"item_2_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021-9258","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hirono, 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Hidenori"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Ishikawa, Yoshihiro"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Yoshioka, Tohru"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-22"}],"displaytype":"detail","filename":"JBiolChem_276_48_45236to42_Kawahara.pdf","filesize":[{"value":"704.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JBiolChem_276_48_45236to42_Kawahara","url":"https://toyama.repo.nii.ac.jp/record/15714/files/JBiolChem_276_48_45236to42_Kawahara.pdf"},"version_id":"8d8cf737-08d2-4987-8d7a-6c8c07c01ea2"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Animals","subitem_subject_scheme":"Other"},{"subitem_subject":"Blotting, Western","subitem_subject_scheme":"Other"},{"subitem_subject":"Calcium","subitem_subject_scheme":"Other"},{"subitem_subject":"Cerebellum","subitem_subject_scheme":"Other"},{"subitem_subject":"Enzyme Activation","subitem_subject_scheme":"Other"},{"subitem_subject":"Immunohistochemistry","subitem_subject_scheme":"Other"},{"subitem_subject":"Isoenzymes","subitem_subject_scheme":"Other"},{"subitem_subject":"Mice","subitem_subject_scheme":"Other"},{"subitem_subject":"Mice, Transgenic","subitem_subject_scheme":"Other"},{"subitem_subject":"Microscopy, Fluorescence","subitem_subject_scheme":"Other"},{"subitem_subject":"Models, Biological","subitem_subject_scheme":"Other"},{"subitem_subject":"Patch-Clamp Techniques","subitem_subject_scheme":"Other"},{"subitem_subject":"Phospholipase C beta","subitem_subject_scheme":"Other"},{"subitem_subject":"Protein Isoforms","subitem_subject_scheme":"Other"},{"subitem_subject":"Protein Kinase C","subitem_subject_scheme":"Other"},{"subitem_subject":"Protein Kinase C beta","subitem_subject_scheme":"Other"},{"subitem_subject":"Protein Kinase C-alpha","subitem_subject_scheme":"Other"},{"subitem_subject":"Purkinje Cells","subitem_subject_scheme":"Other"},{"subitem_subject":"Signal Transduction","subitem_subject_scheme":"Other"},{"subitem_subject":"Time Factors","subitem_subject_scheme":"Other"},{"subitem_subject":"Type C Phospholipases","subitem_subject_scheme":"Other"},{"subitem_subject":"Animals","subitem_subject_scheme":"Other"},{"subitem_subject":"Blotting, Western","subitem_subject_scheme":"Other"},{"subitem_subject":"Calcium","subitem_subject_scheme":"Other"},{"subitem_subject":"Cerebellum","subitem_subject_scheme":"Other"},{"subitem_subject":"Enzyme Activation","subitem_subject_scheme":"Other"},{"subitem_subject":"Immunohistochemistry","subitem_subject_scheme":"Other"},{"subitem_subject":"Isoenzymes","subitem_subject_scheme":"Other"},{"subitem_subject":"Mice","subitem_subject_scheme":"Other"},{"subitem_subject":"Mice, Transgenic","subitem_subject_scheme":"Other"},{"subitem_subject":"Microscopy, Fluorescence","subitem_subject_scheme":"Other"},{"subitem_subject":"Models, Biological","subitem_subject_scheme":"Other"},{"subitem_subject":"Patch-Clamp Techniques","subitem_subject_scheme":"Other"},{"subitem_subject":"Phospholipase C beta","subitem_subject_scheme":"Other"},{"subitem_subject":"Protein Isoforms","subitem_subject_scheme":"Other"},{"subitem_subject":"Protein Kinase C","subitem_subject_scheme":"Other"},{"subitem_subject":"Protein Kinase C beta","subitem_subject_scheme":"Other"},{"subitem_subject":"Protein Kinase C-alpha","subitem_subject_scheme":"Other"},{"subitem_subject":"Purkinje Cells","subitem_subject_scheme":"Other"},{"subitem_subject":"Signal Transduction","subitem_subject_scheme":"Other"},{"subitem_subject":"Time Factors","subitem_subject_scheme":"Other"},{"subitem_subject":"Type C Phospholipases","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Phospholipase Cbeta4 and protein kinase Calpha and/or protein kinase CbetaI are involved in the induction of long term depression in cerebellar Purkinje cells.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Phospholipase Cbeta4 and protein kinase Calpha and/or protein kinase CbetaI are involved in the induction of long term depression in cerebellar Purkinje cells."},{"subitem_title":"Phospholipase Cbeta4 and protein kinase Calpha and/or protein kinase CbetaI are involved in the induction of long term depression in cerebellar Purkinje cells.","subitem_title_language":"en"}]},"item_type_id":"2","owner":"19","path":["558"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-02-22"},"publish_date":"2018-02-22","publish_status":"0","recid":"15714","relation_version_is_last":true,"title":["Phospholipase Cbeta4 and protein kinase Calpha and/or protein kinase CbetaI are involved in the induction of long term depression in cerebellar Purkinje cells."],"weko_creator_id":"19","weko_shared_id":-1},"updated":"2023-07-25T14:30:07.823920+00:00"}