@article{oai:toyama.repo.nii.ac.jp:00015675,
 author = {Eshima, Shizuka and Yokoyama, Satoru and Abe, Takashi and Hayakawa, Yoshihiro and Saiki, Ikuo},
 issue = {6},
 journal = {PLoS ONE},
 month = {Jun},
 note = {application/pdf, Kampo formulations comprise a number of crude natural drugs/herbs as constituents. The crude drugs/herbs have been traditionally classified by their traditional classifications or efficacies in Kampo medicines; however, it has been difficult to establish the scientific link between experimental evidence and traditional classifications in Kampo medicine. To clarify such traditional conceptions, we tested 112 crude drugs/herbs that are major components of Kampo formulations, in the multi-pathway analysis of 10 well-studied transcriptional activities including CREB, ERSF, HIF-1α, IRFs, MYC, NF-κB, p53, SMAD, SOX2, and TCF/LEF in A549 human lung cancer cells. By clustering the results of multi-pathway analysis with the Spearman rank-correlation coefficient and Ward linkage, three distinct traditional categories were significantly enriched in the major groupings, which are heat-clearing and dampness-drying herbs, acrid and warm exterior-resolving herbs, and acrid and cool exterior-resolving herbs. These results indicate that these crude drugs/herbs have similar effects on intracellular signaling and further imply that the traditional classifications of those enriched crude drugs/herbs can be supported by such experimental evidence. Collectively, our new in vitro multi-pathway analysis may be useful to clarify the mechanism of action of crude drugs/herbs and Kampo formulations., Article, PLoS ONE, 2015 Jun 2; 10(6): e0128872},
 title = {Multi-pathway cellular analysis on crude natural drugs/herbs from Japanese Kampo formulations},
 volume = {10},
 year = {2015}
}