{"created":"2023-07-25T09:15:39.162904+00:00","id":15586,"links":{},"metadata":{"_buckets":{"deposit":"0ea32c5c-68f1-4d8e-bb93-4a13769dc50c"},"_deposit":{"created_by":17,"id":"15586","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"15586"},"status":"published"},"_oai":{"id":"oai:toyama.repo.nii.ac.jp:00015586","sets":["439:467:468:469"]},"author_link":["167601","167600","167606","167605","167604","167599","167603","167602"],"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-09-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"13","bibliographicPageEnd":"746","bibliographicPageStart":"740","bibliographicVolumeNumber":"26","bibliographic_titles":[{"bibliographic_title":"NeuroReport"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A novel N-acetyltransferase, Shati/Nat8l, was identified in the nucleus accumbens of mice repeatedly treated with methamphetamine (METH). Shati/Nat8l has been reported to inhibit the pharmacological action induced by METH. Shati/Nat8l produces N-acetylaspartate from aspartate and acetyl-CoA. Previously, we reported that overexpression of Shati/Nat8l in nucleus accumbens attenuates the response to METH by N-acetylaspartylglutamate (which is derived from N-acetylaspartate)-mGluR3 signaling in the mice brain. In the present study, to clarify the type of cells that produce Shati/Nat8l, we carried out in-situ hybridization for the detection of Shati/Nat8l mRNA along with immunohistochemical studies using serial sections of mice brain. Shati/Nat8l mRNA was detected in neuronal cells, but not in astrocytes or microglia cells. Next, we investigated the function of Shati/Nat8l in the neuronal cells in mice brain; then, we used an adeno-associated virus vector containing Shati/Nat8l for transfection and overexpression of Shati/Nat8l protein into the primary cultured neurons to investigate the contribution toward the neuronal activity of Shati/Nat8l. Overexpression of Shati/Nat8l in the mice primary cultured neurons induced axonal growth, but not dendrite elongation at day 1.5 (DIV). This finding indicated that Shati/Nat8l contributes toward neuronal development. LY341495, a selective group II mGluRs antagonist, did not abolish this axonal growth, and N-acetylaspartylglutamate itself did not abolish axon outgrowth in the same cultured system. The cultured neurons overexpressing Shati/Nat8l contained high ATP, suggesting that axon outgrowth is dependent on energy metabolism. This study shows that Shati/Nat8l in the neuron may induce axon outgrowth by ATP synthesis and not through mGluR3 signaling.","subitem_description_type":"Abstract"}]},"item_2_description_40":{"attribute_name":"資源タイプ(DSpace)","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_description_6":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Neuroreport, 2015 Sep 9; 26(13):740-6","subitem_description_type":"Other"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1097/WNR.0000000000000416","subitem_relation_type_select":"DOI"}}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sumi, Kazuyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Uno, Kyosuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsumura, Shohei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miyamoto, Yoshiaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Furukawa-Hibi, Yoko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Muramatsu, Shin-ichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nabeshima, Toshitaka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nitta, Atsumi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-30"}],"displaytype":"detail","filename":"Neuroreport_26(13)_740to746_Sumi.pdf","filesize":[{"value":"214.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Neuroreport_26(13)_740to746_Sumi","url":"https://toyama.repo.nii.ac.jp/record/15586/files/Neuroreport_26(13)_740to746_Sumi.pdf"},"version_id":"fa3bb660-5b37-49f9-a48e-dc3cfe6cfb3b"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-30"}],"displaytype":"detail","filename":"Neuroreport_26(13)_740to746_Sumi_figure.pdf","filesize":[{"value":"814.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Neuroreport_26(13)_740to746_Sumi_figure","url":"https://toyama.repo.nii.ac.jp/record/15586/files/Neuroreport_26(13)_740to746_Sumi_figure.pdf"},"version_id":"7a64cae2-77cf-4f43-9699-3c2bac295003"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Shati/Nat8l","subitem_subject_scheme":"Other"},{"subitem_subject":"NAA","subitem_subject_scheme":"Other"},{"subitem_subject":"NAAG","subitem_subject_scheme":"Other"},{"subitem_subject":"cultured neurons","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Induction of neuronal axon outgrowth by Shati/Nat8l via energy metabolism in mice cultured neurons","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Induction of neuronal axon outgrowth by Shati/Nat8l via energy metabolism in mice cultured neurons"}]},"item_type_id":"2","owner":"17","path":["469"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-01-30"},"publish_date":"2018-01-30","publish_status":"0","recid":"15586","relation_version_is_last":true,"title":["Induction of neuronal axon outgrowth by Shati/Nat8l via energy metabolism in mice cultured neurons"],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2023-07-25T14:34:49.823065+00:00"}