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Authentication of Rhei Rhizoma(Material study)
http://hdl.handle.net/10110/2321
http://hdl.handle.net/10110/23214dee0af9-52ee-4a55-9c0e-273298a4f1ba
名前 / ファイル | ライセンス | アクション |
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wakan05-suppl70.pdf (1.6 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-02-14 | |||||
タイトル | ||||||
タイトル | Authentication of Rhei Rhizoma(Material study) | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Rheum | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Rhei Rhizoma | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | phylogenetic relationship | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | matK gene | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | PCR-RFLP analysis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ARMS analysis | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Komatsu, Katsuko
× Komatsu, Katsuko× YANG, DongYe× Fushimi, Hirotoshi× CAI, ShaoQing |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Rhei Rhizoma (Dahuang in Chinese) is widely known as a purgative and antiinflammatory agent. In the Japanese Pharmacopoeia, Rhei Rhizoma is prescribed for 4 Rheum species, Rheum palmatum, R. tanguticum, R. officinale, and R. coreanum, while the first 3 species are prescribed for Dahuang in the Chinese Pharmacopoeia. Due to the morphologic similarity of the aerial parts, the taxonomy of this genus and the correct identification of Rheum species and their derivative drugs are very difficult. To resolve taxonomic problems of the genus Rheum and develop an ultimate identification method for plants and drugs, molecular analysis of the chloroplast matK gene and nuclear 18S ribosomal RNA gene were performed on 9 species. The sequence comparison of the matK gene revealed that most species had variable sequences not only inter- but also intraspecies. However, the specimens of the same species belonged to the same subclade in the phylogenetic tree constructed based on matK gene sequences, except for R. palmatum, in which specimens belonged to 3 subclades related to their production areas. The nucleotide differences at positions 587,707, and 838 distinguished official species from others, while specific nucleotides at positions 367 and 937 became identification markers for R. palmatum, R. tanguticum, and R. officinale. Moreover, three groups of R. palmatum, each belonging to 3 subclades, were characterized by the nucleotides at positions 619,769,883, and 1061. On the basis of the above marker nucleotides, a convenient and efficient identification method employing Polymerase Chain Reaction-Restriction Fragment Length Polymorphism and Amplification Refractory Mutation System analyses was further developed. The procedure enabled us to identify the botanic origins of 22 drug samples of Rhei Rhizoma. The matK gene sequence was valuable in identifying Rheum species and Rhei Rhizoma and in predicting their production areas, and could be used as an index for quality evaluation of Rhei Rhizoma. | |||||
書誌情報 |
和漢医薬学雑誌 = Journal of traditional medicines 巻 22, 号 suppl., p. 70-85, 発行日 2005 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 18801447 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12035198 | |||||
権利 | ||||||
権利情報 | rights: 本文データは和漢医薬学会の許諾に基づきCiNiiから複製したものである | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | 和漢医薬学会 | |||||
資源タイプ(DSpace) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article |