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Transformation of shikonin by a cell-free extract of Eubacterium sp. A-44, a human intestinal bacterium
http://hdl.handle.net/10110/1945
http://hdl.handle.net/10110/19455ef2afa5-0359-43b8-bbc2-32a85978edd4
名前 / ファイル | ライセンス | アクション |
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20010203.pdf (790.5 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-01-24 | |||||
タイトル | ||||||
タイトル | Transformation of shikonin by a cell-free extract of Eubacterium sp. A-44, a human intestinal bacterium | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Eubacterium | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | human intestinal bacteria | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | metabolism | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | prometaboshikonin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | shikonin | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
MESELHY, MeselhyR.
× MESELHY, MeselhyR.× Nishimoto, Eri× Akao, Teruaki× Hattori, Masao |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | For the purpose of investigating the metabolic processes of shikonin (1) by human intestinal bacteria, we prepared a sonicated bacterial cell suspension and a crude enzyme preparation from Eubacterium sp. A-44, one of the intestinal bacteria capable of transforming 1 to various metabolites. After anaerobic incubation with the suspension for 1 hr, most of 1 was transformed to prometaboshi-konin (2), and metaboshikonins I (3) and II (4). However, under aerobic conditions, the dimers, shikometabolins A (5) and B (6), were predominantly formed. In the presence of the crude enzyme preparation, formation of 2-4 was inhibited by oxygen, but markedly enhanced by the addition of NADH. On the other hand, formation of 5 and 6 was appreciably accelerated by the addition of NAD^+. In the absence of the crude enzyme preparation, NADH and/or NAD^+ showed no ability to transform 1 to the any metabolites, as in the case of a thermally inactivated preparation. Accordingly, the two different metabolic processes leading to compounds 2-4 and com-pounds 5 and 6 by Eubacterium sp. A-44 are concluded to be enzyme-dependent in the presence of NADH and NAD^+. ヒト腸内細菌による紫根成分shikoninの代謝過程を研究する目的でshikonin(1)を種々の代謝物に変換する腸内細菌Eubacterium sp.A-44の細胞破砕懸濁液及び粗酵素標品を調製した。この粗酵素標品とshikonin(1)を嫌気条件下で1時間インキュベーションすると,shikonin(1)のほとんどはprometaboshikonin(2)およびmetaboshikonin I(3),II(4)に変換された。しかしながら,好気条件下ではshikometabolin A(5),B(6)などの二量体が生成した。化合物2-4の生成は,酸素によって阻害され,NADHの添加により増強した。一方,化合物5,6の生成はNAD^+の添加により増加した。加熱処理した場合と同様,粗酵素標品を除去した場合は,NADH,NAD^+を添加してもshikoninの変換は起こらなかった。したがって,NADHやNAD^+の存在下での化合物2-4や化合物5,6への変換過程は酵素反応によって触媒されることを示している。 |
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書誌情報 |
和漢医薬学雑誌 = Journal of traditional medicines 巻 18, 号 2, p. 58-63, 発行日 2001-04 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13406302 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN10486121 | |||||
権利 | ||||||
権利情報 | rights: 本文データは和漢医薬学会の許諾に基づき複製したものである | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | 和漢医薬学会 | |||||
資源タイプ(DSpace) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article |