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Autocrine/Paracrine Mechanism of Insulin like Growth Factor-1 secretion, and the Effect of IGF-1 on Proteoglycan Synthesis in Bovine Intervertebral Discs
https://doi.org/10.11501/3108972
https://doi.org/10.11501/3108972ee4e5e18-8410-44c8-acb7-d4e398e612b2
名前 / ファイル | ライセンス | アクション |
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000000293710.pdf (6.7 MB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2013-02-07 | |||||
タイトル | ||||||
タイトル | Autocrine/Paracrine Mechanism of Insulin like Growth Factor-1 secretion, and the Effect of IGF-1 on Proteoglycan Synthesis in Bovine Intervertebral Discs | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | insulin like growth factor-1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | autocrine/paracrine mechanism | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | intervertebral disc | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
ID登録 | ||||||
ID登録 | 10.11501/3108972 | |||||
ID登録タイプ | JaLC | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
著者 |
Osada, Ryusuke
× Osada, Ryusuke |
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著者別名 | ||||||
姓名 | 長田, 龍介 | |||||
その他(別言語等)のタイトル | ||||||
その他のタイトル | 牛尾椎椎間板におけるインスリン様成長因子-1(IGF-1)の自己分泌機構及びIGF-1が椎間板のプロテオグリカン合成に与える影響 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The present study was undertaken to investigate the effect of IGF-1 on proteoglycan (PG) synthesis and the autocrine/paracrine mechanisms involving insulin-like growth factor-1 (IGF-1) in the bovine coccygeal intervertebral disc. IGF-1 stimulated PG synthesis in cultured cells of nucleus pulposus of bovine intervertebral discs in a dose-dependent manner, and the effect was inhibited by an anti-IGF-1 monoclonal antibody. Insitu hybridization histochemistry revealed the expression of IGF-1 mRNA in the cultured cells, and IGF-1 production in these cells was demonstrated by radioimmunoassay. IGF-1 receptor in the cultured cells was also immunohistochemically demonstrated. The Scatchard analysis using an ^<125>I-IGF-1 binding assay showed that the cells cultured in monolayer had a single type of IGF-1 receptor, whose affinity and number were estimated to be 7.38 x 10^8/M and 9.27 x 10^4/cell, respectively. These results suggest that IGF-1 stimulates PG synthesis in cells of the nucleus pulposus, and that these cells in culture have an IGF-1 autocrine/paracrine mechanism. The expressions of IGF-1 mRNA and IGF-1 receptor in disc tissue were greater in cells of the nucleus pulposus of fetal bovine intervertebral discs than in those of the adult discs. These findings suggest that the autocrine/paracrine IGF-1 action is more active in cells of young nucleus pulposus than in cells of matured subjects. |
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内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 富山医科薬科大学・博士(医学)・甲第240号・長田龍介・1996/3/8 | |||||
書誌情報 | 発行日 1996-03-08 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
資源タイプ(DSpace) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
学位名 | ||||||
学位名 | 博士(医学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 13202 | |||||
学位授与機関名 | 富山医科薬科大学 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 1996-03-08 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲第240号 |