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Induction of neuronal axon outgrowth by Shati/Nat8l via energy metabolism in mice cultured neurons
http://hdl.handle.net/10110/00018051
http://hdl.handle.net/10110/000180516190c50d-487e-44fe-b59c-d3e48eb14c75
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Neuroreport_26(13)_740to746_Sumi (214.0 kB)
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Neuroreport_26(13)_740to746_Sumi_figure (814.1 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-01-30 | |||||
タイトル | ||||||
タイトル | Induction of neuronal axon outgrowth by Shati/Nat8l via energy metabolism in mice cultured neurons | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Shati/Nat8l | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | NAA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | NAAG | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cultured neurons | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Sumi, Kazuyuki
× Sumi, Kazuyuki× Uno, Kyosuke× Matsumura, Shohei× Miyamoto, Yoshiaki× Furukawa-Hibi, Yoko× Muramatsu, Shin-ichi× Nabeshima, Toshitaka× Nitta, Atsumi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A novel N-acetyltransferase, Shati/Nat8l, was identified in the nucleus accumbens of mice repeatedly treated with methamphetamine (METH). Shati/Nat8l has been reported to inhibit the pharmacological action induced by METH. Shati/Nat8l produces N-acetylaspartate from aspartate and acetyl-CoA. Previously, we reported that overexpression of Shati/Nat8l in nucleus accumbens attenuates the response to METH by N-acetylaspartylglutamate (which is derived from N-acetylaspartate)-mGluR3 signaling in the mice brain. In the present study, to clarify the type of cells that produce Shati/Nat8l, we carried out in-situ hybridization for the detection of Shati/Nat8l mRNA along with immunohistochemical studies using serial sections of mice brain. Shati/Nat8l mRNA was detected in neuronal cells, but not in astrocytes or microglia cells. Next, we investigated the function of Shati/Nat8l in the neuronal cells in mice brain; then, we used an adeno-associated virus vector containing Shati/Nat8l for transfection and overexpression of Shati/Nat8l protein into the primary cultured neurons to investigate the contribution toward the neuronal activity of Shati/Nat8l. Overexpression of Shati/Nat8l in the mice primary cultured neurons induced axonal growth, but not dendrite elongation at day 1.5 (DIV). This finding indicated that Shati/Nat8l contributes toward neuronal development. LY341495, a selective group II mGluRs antagonist, did not abolish this axonal growth, and N-acetylaspartylglutamate itself did not abolish axon outgrowth in the same cultured system. The cultured neurons overexpressing Shati/Nat8l contained high ATP, suggesting that axon outgrowth is dependent on energy metabolism. This study shows that Shati/Nat8l in the neuron may induce axon outgrowth by ATP synthesis and not through mGluR3 signaling. | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Neuroreport, 2015 Sep 9; 26(13):740-6 | |||||
書誌情報 |
NeuroReport 巻 26, 号 13, p. 740-746, 発行日 2015-09-09 |
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DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1097/WNR.0000000000000416 | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
資源タイプ(DSpace) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article |