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Characterization of anti-herpes simplex virus type 1 activity of an alkaloid FK 3000 from Stephania cepharantha
http://hdl.handle.net/10110/1915
http://hdl.handle.net/10110/19154c1e1816-3870-4a17-9fce-9a809e5f76cf
名前 / ファイル | ライセンス | アクション |
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20020402.pdf (1.4 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-01-23 | |||||
タイトル | ||||||
タイトル | Characterization of anti-herpes simplex virus type 1 activity of an alkaloid FK 3000 from Stephania cepharantha | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | herpes simplex virus | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Stephania cepharantha | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | FK 3000 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | antiviral activity | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Hattori, Masao
× Hattori, Masao× Ohsaki, Motoki× Kurokawa, Masahiko× NAWAWI, As'ari× Nakamura, Norio× Shiraki, Kimiyasu |
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著者別名 | ||||||
姓名 | 服部, 征雄 | |||||
著者別名 | ||||||
姓名 | 白木, 公康 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A morphinane alkaloid FK 3000 (6,7-di-O-acetylsinococuline) from the root tubers of Stephania cepharantha showed antiviral activity against acyclovir (ACV)- and phosphonoacetic acid (PAA)-resistant herpes simplex virus type 1 (HSV-1), influenza virus, measles virus, and poliovirus. The anti-HSV action of FK 3000 was assessed in comparison with that of PAA that inhibits the activity of HSV DNA polymerase and HSV DNA synthesis. FK 3000 inhibited the growth of thymidine kinase-deficient and ACV and PAA-resistant HSV-1 strains, as well as wild type HSV strains in Vero cells. This compound, as well as PAA, interfered with the synthesis of late viral proteins but not early viral proteins. The analysis of HSV DNA synthesis by slot blot hybridization showed that FK 3000 inhibited the viral DNA synthesis in a dose-dependent manner. However, the viral RNA was partially synthesized in the presence of FK 3000 (even at a dose that HSV DNA synthesis was inhibited) and PAA, indicating that FK 3000, as well as PAA, allowed early viral RNA synthesis but not viral DNA synthesis. Since partially purified HSV DNA polymerase activity was not inhibited by FK 3000, this compound was suggested to inhibit HSV DNA synthesis by a mechanism different from that of PAA. Stephania cepharantha(タマザキツヅラフジ)から得たモルフィン骨格を有するアルカロイドFK3000はacyclovirやphosphonoacetic acid(PAA)抵抗性を有するHSV-1, influenza virs, measles virus, poho virsに対しても抗ウイルス作用を有していた。この抗HSV作用をHSV DNA polymeraseを阻害することによりHSV DNA合成を阻害することが知られているPAAとの比較から検討した。HSVに感染したVero細胞においてFK3000は,PAAと同様に後期ウイルス蛋白の合成を阻害したが初期ウイルス蛋白には影響しなかった。Slot blot hybridization法でHSV DNA合成を調べると,FK3000は濃度依存的にウイルスDNA合成を阻害することが判明した。しかし,ウイルスRNA合成はHSV DNA合成が阻害される濃度でもFK3000およびPAAによって部分的にのみ阻害された。このことはPAAと同様にFK3000はウイルスDNA合成は阻害するが初期ウイルスRNAの合成は許容することを示している。FK3000は粗精製したHSV-DNA polymerase活性を阻害しないことから,PAAと異なった機構でHSV DNA合成を阻害していることが示唆された。 |
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書誌情報 |
和漢医薬学雑誌 = Journal of traditional medicines 巻 19, 号 4, p. 129-136, 発行日 2002-08 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13406302 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN10486121 | |||||
権利 | ||||||
権利情報 | rights: 本文データは和漢医薬学会の許諾に基づき複製したものである | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | 和漢医薬学会 | |||||
資源タイプ(DSpace) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article |