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K+-Cl- Cotransporter-3a Up-regulates Na+,K+-ATPase in Lipid Rafts of Gastric Luminal Parietal Cells*
http://hdl.handle.net/10110/2525
http://hdl.handle.net/10110/25258c1f05fa-850c-40c4-b521-8c5331b8fec6
名前 / ファイル | ライセンス | アクション |
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JBC-yakubutsuseirigaku.pdf (1.6 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-04-22 | |||||
タイトル | ||||||
タイトル | K+-Cl- Cotransporter-3a Up-regulates Na+,K+-ATPase in Lipid Rafts of Gastric Luminal Parietal Cells* | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Fujii, Takuto
× Fujii, Takuto× Takahashi, Yuji× Itomi, Yasuo× Fujita, Kyosuke× Morii, Magothoshi× Tabuchi, Yoshiaki× Asano, Shinji× Tsukada, Kazuhiro× Takeguchi, Noriaki× Sakai, Hideki |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Gastric parietal cells migrate from the luminal to the basal region of the gland and they gradually lose acid secretory activity. So far, distribution and function of K+-Clcotransporters (KCCs) in gastric parietal cells have not been reported. We found that KCC3a but not KCC3b mRNA was highly expressed and KCC3a protein was predominantly expressed in the basolateral membrane of rat gastric parietal cells located in the luminal region of the glands. KCC3a and Na+,K+-ATPase α1-subunit (α1NaK) were co-immunoprecipitated and both of them were highly localized in a lipid raft fraction. The ouabain-sensitive K+-dependent ATP-hydrolyzing activity (Na+,K+-ATPase activity) was significantly inhibited by a KCC inhibitor (DIOA). The stable exogenous expression of KCC3a in LLC-PK1 cells resulted in association of KCC3a with endogenous α1NaK and it recruited α1NaK in lipid rafts, accompanying increases of Na+,K+-ATPase activity and ouabain-sensitive Na+ transport activity which were suppressed by DIOA, while the total expression level of α1NaK in the cells was not significantly altered. On the other hand, the expression of KCC4 induced no association with α1NaK. In conclusion, KCC3a forms a functional complex with α1NaK in the basolateral membrane of luminal parietal cells and it up-regulates α1NaK in lipid rafts, while KCC3a is absent in basal parietal cells. | |||||
書誌情報 |
Journal of Biological Chemistry 巻 283, 号 11, p. 6869-6977, 発行日 2008-03-14 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 219258 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00251083 | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
NIIサブジェクト | ||||||
主題Scheme | Other | |||||
主題 | 薬学 | |||||
出版者 | ||||||
出版者 | The American Society for Biochemistry and Molecular Biology | |||||
資源タイプ(DSpace) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article |